Journal: Neurobiology of Stress

Article Title: The insular cortex-nucleus tractus solitarius glutamatergic pathway involved in acute stress-induced gastric mucosal damage in rats

doi: 10.1016/j.ynstr.2025.100723

Figure Lengend Snippet: Inhibition IC glutamate neurons to the influence of the gastric mucosa damage induced by RWIS. A: Schematic of virus injection; B–C: Viral expression in the left and right IC (40 × ) scale bar, 200 μm; D–E: Viral expression in the left and right IC (100 × ) scale bar, 100 μm; F: c-Fos expression in RWIS rats treated with CaMKIIa-mCherry in the IC; G–H: c-Fos expression in RWIS rats in the IC of the CaMKIIa-hm4d + saline- or CNO-injected groups (100 × ). scale bar,100 μm, c-Fos in a rectangle were magnified to a higher magnification (400 × ); scale bar, 20 μm; I: The number of c-Fos-IR neurons in the IC was quantified. compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm4d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); J: Gastric mucosal damage in RWIS rats treated with CaMKIIa-mCherry; K–L: Gastric mucosal damage in RWIS rats in the IC of the CaMKIIa-hm4d + saline- or CNO-injected groups; M: Quantification of erosion index (EI). compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.0, compared with hm4d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); N: Western blot of Occludin, Claudin-1, PCNA, NOS and CHAT protein expression in different groups; O–S: Quantification of the relative average grayscale value of Occludin/β-actin, Claudin-1/β-actin, PCNA/β-actin, NOS/β-actin and CHAT/β-actin. compared with the mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm4d + SA group, #: P < 0.05, ##: P < 0.01 (n = 5).

Article Snippet: After incubation at 4 °C overnight, rabbit anti-β-actin (1:8000; Huabio; Hangzhou, China), rabbit Occludin (1:1000; Huabio; Hangzhou, China), mouse anti- Claudin-1 (1:1000; Huabio; Hangzhou, China), rabbit anti-PCNA(1:5000; Proteintech,USA), rabbit anti-nNOS(1:500; Huabio; Hangzhou, China), rabbit anti-CHAT(1:500; Huabio; Hangzhou, China), rabbit anti-PSD95 (1:1000; Huabio; Hangzhou, China), rabbit anti-SYN-1 (1:1000; Huabio; Hangzhou, China).

Techniques: Inhibition, Virus, Injection, Expressing, Saline, Western Blot

Journal: Neurobiology of Stress

Article Title: The insular cortex-nucleus tractus solitarius glutamatergic pathway involved in acute stress-induced gastric mucosal damage in rats

doi: 10.1016/j.ynstr.2025.100723

Figure Lengend Snippet: Activation IC glutamate neurons to the influence of the gastric mucosa damage induced by RWIS. A: Schematic of virus injection; B–C Viral expression in the left and right IC (40 × ) scale bar, 200 μm; D–E: Viral expression in the left and right IC (100 × ) scale bar,100 μm; F: c-Fos expression in RWIS rats treated with CaMKIIa-mCherry in the IC; G–H: c-Fos expression in RWIS rats in the IC of the CaMKIIa-hm3d + saline- or CNO-injected groups (100 × ) scale bar,100 μm, c-Fos in a rectangle were magnified to a higher magnification (400 × ); scale bar, 20 μm; I: The number of c-Fos-IR neurons in the IC was quantified. compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm3d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); J: Gastric mucosal damage in RWIS rats treated with CaMKIIa-mCherry; K–L: Gastric mucosal damage in RWIS rats in the IC of the CaMKIIa-hm3d + saline- or CNO-injected groups; M: Quantification of erosion index (EI). compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.0, compared with hm3d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); N: Western blot of Occludin, Claudin-1, PCNA, NOS and CHAT protein expression in different groups; O–S: Quantification of the relative average grayscale value of Occludin/β-actin, Claudin-1/β-actin, PCNA/β-actin, NOS/β-actin and CHAT/β-actin. compared with the mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm3d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6).

Article Snippet: After incubation at 4 °C overnight, rabbit anti-β-actin (1:8000; Huabio; Hangzhou, China), rabbit Occludin (1:1000; Huabio; Hangzhou, China), mouse anti- Claudin-1 (1:1000; Huabio; Hangzhou, China), rabbit anti-PCNA(1:5000; Proteintech,USA), rabbit anti-nNOS(1:500; Huabio; Hangzhou, China), rabbit anti-CHAT(1:500; Huabio; Hangzhou, China), rabbit anti-PSD95 (1:1000; Huabio; Hangzhou, China), rabbit anti-SYN-1 (1:1000; Huabio; Hangzhou, China).

Techniques: Activation Assay, Virus, Injection, Expressing, Saline, Western Blot

Journal: Neurobiology of Stress

Article Title: The insular cortex-nucleus tractus solitarius glutamatergic pathway involved in acute stress-induced gastric mucosal damage in rats

doi: 10.1016/j.ynstr.2025.100723

Figure Lengend Snippet: Effect of inhibition of the IC-NTS glutamatergic pathway on RWIS-induced gastric mucosal damage. A: Schematic of virus injection; B: Viral expression in the IC (40 × ) scale bar, 200 μm; C: Viral expression in the IC (100 × ) scale bar,100 μm; D: c-Fos expression in RWIS rats treated with CaMKIIa-mCherry in the IC; E–F: c-Fos expression in RWIS rats in the IC of the CaMKIIa-hm4d + saline- or CNO-injected groups (100 × ). scale bar,100 μm, c-Fos in a rectangle were magnified to a higher magnification (400 × ); scale bar, 20 μm; G: The number of c-Fos-IR neurons in the insul was quantified. compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm4d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); H: c-Fos expression in RWIS rats treated with CaMKIIa-mCherry in the NTS; I–J: c-Fos expression in RWIS rats in the NTS of the CaMKIIa-hm4d + saline- or CNO-injected groups (100 × ). scale bar,100 μm; K: The number of c-Fos-IR neurons in the NTS was quantified. compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm4d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); L: Gastric mucosal damage in RWIS rats treated with CaMKIIa-mCherry; M–N: Gastric mucosal damage in RWIS rats in the IC of the CaMKIIa-hm4d + saline- or CNO-injected groups; O: Quantification of erosion index (EI). compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.0, compared with hm4d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); P: Western blot of Occludin, Claudin-1, PCNA, NOS and CHAT protein expression in different groups; Q–U: Quantification of the relative average grayscale value of Occludin/β-actin, Claudin-1/β-actin, PCNA/β-actin, NOS/β-actin and CHAT/β-actin. compared with the mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm4d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6).

Article Snippet: After incubation at 4 °C overnight, rabbit anti-β-actin (1:8000; Huabio; Hangzhou, China), rabbit Occludin (1:1000; Huabio; Hangzhou, China), mouse anti- Claudin-1 (1:1000; Huabio; Hangzhou, China), rabbit anti-PCNA(1:5000; Proteintech,USA), rabbit anti-nNOS(1:500; Huabio; Hangzhou, China), rabbit anti-CHAT(1:500; Huabio; Hangzhou, China), rabbit anti-PSD95 (1:1000; Huabio; Hangzhou, China), rabbit anti-SYN-1 (1:1000; Huabio; Hangzhou, China).

Techniques: Inhibition, Virus, Injection, Expressing, Saline, Western Blot

Journal: Neurobiology of Stress

Article Title: The insular cortex-nucleus tractus solitarius glutamatergic pathway involved in acute stress-induced gastric mucosal damage in rats

doi: 10.1016/j.ynstr.2025.100723

Figure Lengend Snippet: Effect of activation of the IC-NTS glutamatergic pathway on RWIS-induced gastric mucosal damage. A: Schematic of virus injection; B: viral expression in the IC (40 × ) scale bar, 200 μm; C: viral expression in the IC (100 × ) scale bar,100 μm; D: c-Fos expression in RWIS rats treated with CaMKIIa-mCherry in the IC; E–F: c-Fos expression in RWIS rats in the IC of the CaMKIIa-hm3d + saline- or CNO-injected groups (100 × ). scale bar,100 μm, c-Fos in a rectangle were magnified to a higher magnification (400 × ); scale bar, 20 μm; G: The number of c-Fos-IR neurons in the insul was quantified. compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm3d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); H: c-Fos expression in RWIS rats treated with CaMKIIa-mCherry in the NTS; I–J: c-Fos expression in RWIS rats in the NTS of the CaMKIIa-hm3d + saline- or CNO-injected groups (100 × ). scale bar,100 μm; K: The number of c-Fos-IR neurons in the NTS was quantified. compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm3d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); L: Gastric mucosal damage in RWIS rats treated with CaMKIIa-mCherry; M–N: Gastric mucosal damage in RWIS rats in the IC of the CaMKIIa-hm3d + saline- or CNO-injected groups; O: Quantification of erosion index (EI). compared with mCherry group, ∗: P < 0.05, ∗∗: P < 0.0, compared with hm3d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6); P: Western blot of Occludin, Claudin-1, PCNA, NOS and CHAT protein expression in different groups; Q–U: Quantification of the relative average grayscale value of Occludin/β-actin, Claudin-1/β-actin, PCNA/β-actin, NOS/β-actin and CHAT/β-actin. compared with the mCherry group, ∗: P < 0.05, ∗∗: P < 0.01, compared with hm3d + SA group, #: P < 0.05, ##: P < 0.01 (n = 6).

Article Snippet: After incubation at 4 °C overnight, rabbit anti-β-actin (1:8000; Huabio; Hangzhou, China), rabbit Occludin (1:1000; Huabio; Hangzhou, China), mouse anti- Claudin-1 (1:1000; Huabio; Hangzhou, China), rabbit anti-PCNA(1:5000; Proteintech,USA), rabbit anti-nNOS(1:500; Huabio; Hangzhou, China), rabbit anti-CHAT(1:500; Huabio; Hangzhou, China), rabbit anti-PSD95 (1:1000; Huabio; Hangzhou, China), rabbit anti-SYN-1 (1:1000; Huabio; Hangzhou, China).

Techniques: Activation Assay, Virus, Injection, Expressing, Saline, Western Blot